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Volume 12, Issue 3 (7-2024)
J. Pediatr. Rev 2024, 12(3): 273-282 |
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Hosseinzadeh F, Rahimzadeh G, Ahangarkani F, Salehpour S M, Rezai S, Valadan R, et al . The Emergence of Multi-drug-resistant and Extensively-drug-resistant Pseudomonas aeruginosa After the COVID‑19 Pandemic in North Iran. J. Pediatr. Rev 2024; 12 (3) :273-282
URL: http://jpr.mazums.ac.ir/article-1-647-en.html
URL: http://jpr.mazums.ac.ir/article-1-647-en.html
Fatemeh Hosseinzadeh1 
, Golnar Rahimzadeh1 , Fatemeh Ahangarkani2 , Seyedeh Mahsa Salehpour3 , Shaghayegh Rezai4 , Reza Valadan5 , Laleh Vahedi6 , Somayeh Sheidaei6 , Faezeh Sadat Movahedi1 , Raha Rezai1 , Mohammad Sadegh Rezai * 7
, Golnar Rahimzadeh1 , Fatemeh Ahangarkani2 , Seyedeh Mahsa Salehpour3 , Shaghayegh Rezai4 , Reza Valadan5 , Laleh Vahedi6 , Somayeh Sheidaei6 , Faezeh Sadat Movahedi1 , Raha Rezai1 , Mohammad Sadegh Rezai * 7
1- Pediatric Infectious Diseases Research Center, Communicable Diseases Institute, Mazandaran University of Medical Sciences, Sari, Iran.
2- Antimicrobial Resistance Research Center, Communicable Diseases Institute, Mazandaran University of Medical Sciences, Sari, Iran.
3- Students Research Committee, Mazandaran University of Medical Sciences, Sari, Iran.
4- Department of Medical Microbiology and Virology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
5- Molecular and Cell Biology Research Center, Mazandaran University of Medical Sciences, Sari, Iran.
6- Department of Pathology, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran.
7- Pediatric Infectious Diseases Research Center, Communicable Diseases Institute, Mazandaran University of Medical Sciences, Sari, Iran. ,drmsrezaii@yahoo.com
2- Antimicrobial Resistance Research Center, Communicable Diseases Institute, Mazandaran University of Medical Sciences, Sari, Iran.
3- Students Research Committee, Mazandaran University of Medical Sciences, Sari, Iran.
4- Department of Medical Microbiology and Virology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
5- Molecular and Cell Biology Research Center, Mazandaran University of Medical Sciences, Sari, Iran.
6- Department of Pathology, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran.
7- Pediatric Infectious Diseases Research Center, Communicable Diseases Institute, Mazandaran University of Medical Sciences, Sari, Iran. ,
Abstract: (553 Views)
Background: During the COVID-19 pandemic, the excessive use of antibiotics to manage co-infections and reduce mortality rates may have contributed to the increase in antimicrobial resistance.
Objectives: This research was conducted to evaluate the antibiotic resistance patterns of multidrug-resistant (MDR) Pseudomonas aeruginosa strains during the COVID-19 pandemic in North Iran.
Methods: This cross-sectional research was conducted at four teaching hospitals in Sari, Iran between May 2022 and June 2023. The macro dilution broth technique was employed to determine the minimum inhibitory concentration (MIC). All isolates were screened using the multiplex polymerase chain reaction (PCR) method for blaAmpC, blaCTX, blaSHV, blaSIM, blaSPM, blaIMP, and also virulence genes, including TOXA and ExoS.
Results: The antibiotics co-amoxiclav and gentamicin showed the least activity in terms of MIC50 values, while meropenem exhibited the most potent activity based on the geometric mean of minimum inhibitory concentration (GM MIC) values. The multiplex PCR method revealed that all isolates possessed the ExoS toxin gene, while the ExoA gene was not detected. The observed frequencies of resistance genes were blaSHV (91.3%), blaCTX-M (76%), blaAmpC (91.3%), and blaIMP (95.2%). The blaSPM and blaSIM genes were not distinguished.
Conclusions: This research recommends piperacillin/tazobactam and meropenem for the treatment of multi-drug-resistant P. aeruginosa infections.
Objectives: This research was conducted to evaluate the antibiotic resistance patterns of multidrug-resistant (MDR) Pseudomonas aeruginosa strains during the COVID-19 pandemic in North Iran.
Methods: This cross-sectional research was conducted at four teaching hospitals in Sari, Iran between May 2022 and June 2023. The macro dilution broth technique was employed to determine the minimum inhibitory concentration (MIC). All isolates were screened using the multiplex polymerase chain reaction (PCR) method for blaAmpC, blaCTX, blaSHV, blaSIM, blaSPM, blaIMP, and also virulence genes, including TOXA and ExoS.
Results: The antibiotics co-amoxiclav and gentamicin showed the least activity in terms of MIC50 values, while meropenem exhibited the most potent activity based on the geometric mean of minimum inhibitory concentration (GM MIC) values. The multiplex PCR method revealed that all isolates possessed the ExoS toxin gene, while the ExoA gene was not detected. The observed frequencies of resistance genes were blaSHV (91.3%), blaCTX-M (76%), blaAmpC (91.3%), and blaIMP (95.2%). The blaSPM and blaSIM genes were not distinguished.
Conclusions: This research recommends piperacillin/tazobactam and meropenem for the treatment of multi-drug-resistant P. aeruginosa infections.
Keywords: COVID-19, Drug resistance, Pseudomonas aeruginosa, Healthcare associated infections, Multiplex polymerase chain reaction (PCR)
Type of Study: Original Article |
Subject:
Microbiology
Received: 2024/06/20 | Accepted: 2024/09/1 | Published: 2024/07/31
Received: 2024/06/20 | Accepted: 2024/09/1 | Published: 2024/07/31
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